BD-PB1543-Mouse Nlrp6 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

Mouse Nlrp6 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB1543

产品名称

Mouse Nlrp6 Rabbit Polyclonal Antibody (Center)

别名

NACHT, LRR and PYD domains-containing protein 6, Angiotensin II/vasopressin receptor, PYRIN-containing APAF1-like protein 5-like, Nlrp6, Nalp6, Pypaf5

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Swissprot No.

Q91WS2

Mouse Gene ID

NP_598707.2

特异性

This Mouse Nlrp6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-372 amino acids from the Central region of mouse Nlrp6.

稀释度

IHC-P-Leica~~1:500;WB~~1:1000

预测分子量

97kDa

同种型

Rabbit Ig

背景介绍

Nlrp6 may mediate activation of CASP1 via ASC and promote activation of NF-kappa-B (By similarity).

组织表达

Highly expressed in the gastrointestinal tract, predominantly in colonic myofibroblasts and in colonic epithelial and endothelial cells. Within the intestinal mucosa, highly expressed by goblet cells. Also expressed in hepatocytes and in immune cells, including CD4(+) and CD8(+) T-cells, dendritic cells, mastocytes and peritoneal macrophages, as well as in lung, kidney, bladder and gonads

细胞定位

[Isoform 1]: Cytoplasm {ECO:0000250|UniProtKB:Q63035}. Inflammasome {ECO:0000250|UniProtKB:P59044}. Cell membrane {ECO:0000250|UniProtKB:Q63035}. Nucleus membrane {ECO:0000250|UniProtKB:Q63035}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using BD-PB1543 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using BD-PB1543 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-Mouse Nlrp6 Antibody (Center) at 1:500 dilution Lane 1: Mouse small intestine lysate Lane 2: Mouse heart lysate Lane 3: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-Mouse Nlrp6 Antibody (Center) at 1:1000 dilution Lane 1: Mouse small intestine lysate Lane 2: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-Mouse Nlrp6 Antibody (Center) at 1:500 dilution Lane 1: Mouse small intestine lysate Lane 2: Mouse liver lysate Lane 3: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-NLRP6 Antibody at 1:2000 dilution + mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Mouse Nlrp6 Antibody western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Nlrp6 antibody detected the Nlrp6 protein (arrow).