产品名称
ALKB2 Rabbit Polyclonal Antibody
蛋白名称
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 (EC 1.14.11.33) (Alkylated DNA repair protein alkB homolog 2) (DNA oxidative demethylase ALKBH2) (Oxy DC1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q6NS38
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q6NS38/entry
Mouse Swissprot No.
Q6P6J4
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6P6J4
免疫原
Synthesized peptide derived from human protein . at AA range: 140-220
特异性
ALKB2 Polyclonal Antibody detects endogenous levels of protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008],
组织表达
Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.
细胞定位
Nucleus . Nucleus, nucleolus . Nucleus, nucleoplasm . Relocates to the replication foci during S-phase. .
功能
cofactor:Binds 1 Fe(2+) ion per subunit.,enzyme regulation:Activated by ascorbate.,function:Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.,similarity:Belongs to the alkB family.,subcellular location:Detected in replication foci during S-phase.,tissue specificity:Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
