BD-PB1454-Hippocalcin Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Hippocalcin Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1454

产品名称

Hippocalcin Rabbit Polyclonal Antibody (N-term)

别名

Neuron-specific calcium-binding protein hippocalcin, Calcium-binding protein BDR-2, HPCA, BDR2

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_002134.2

Human Swissprot No.

P84074

特异性

This Hippocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human Hippocalcin.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

22kDa

同种型

Rabbit Ig

背景介绍

Hippocalcin is a member of neuron-specific calcium-binding proteins family found in the retina and brain. This protein is associated with the plasma membrane. It has similarities to proteins located in the photoreceptor cells that regulate photosignal transduction in a calcium-sensitive manner. This protein displays recoverin activity and a calcium-dependent inhibition of rhodopsin kinase. It is identical to the rat and mouse hippocalcin proteins and thought to play an important role in neurons of the central nervous system in a number of species.

组织表达

Brain specific..

细胞定位

Cytoplasm, cytosol {ECO:0000250|UniProtKB:P84076, ECO:0000269|PubMed:28398555}. Membrane {ECO:0000250|UniProtKB:P84076}; Peripheral membrane protein {ECO:0000250|UniProtKB:P84076} Note=Association with membranes is calcium-dependent (By similarity) Enriched in the perinuclear region, probably at the trans Golgi network in response to calcium (PubMed:28398555) {ECO:0000250|UniProtKB:P84076, ECO:0000269|PubMed:28398555}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Western blot analysis of lysate from human brain tissue lysate, using Hippocalcin Antibody (R17) . BD-PB1454 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human brain tissue reacted with Hippocalcin antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.