产品名称
CDC45 Rabbit Polyclonal Antibody
基因名称
CDC45 CDC45L CDC45L2 UNQ374/PRO710
蛋白名称
Cell division control protein 45 homolog (PORC-PI-1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O75419
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O75419/entry
Mouse Swissprot No.
Q9Z1X9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9Z1X9
免疫原
Synthesized peptide derived from human protein . at AA range: 90-170
特异性
CDC45 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
组织表达
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;
功能
developmental stage:Transcript peaks at G1-S transition, but total protein remains constant throughout the cell cycle. Expressed in multiple tissues during embryogenesis, including neural crest-derived structures.,function:Required for initiation of chromosomal DNA replication.,similarity:Belongs to the CDC45 family.,subunit:Associated with ORC2L.,tissue specificity:Widely expressed, highest levels are found in adult testis and tyhmus and in fetal liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
