BD-PB1450-Connexin 36 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Connexin 36 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB1450

产品名称

Connexin 36 Rabbit Polyclonal Antibody (C-term)

别名

Gap junction delta-2 protein, Connexin-36, Cx36, Gap junction alpha-9 protein, GJD2, GJA9

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_065711.1

Human Swissprot No.

Q9UKL4

特异性

This Connexin 36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-321 amino acids from the C-terminal region of human Connexin 36.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

36kDa

同种型

Rabbit Ig

背景介绍

GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.

组织表达

Highly expressed in neurons.

细胞定位

Cell membrane; Multi-pass membrane protein. Cell junction, gap junction

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

All lanes : Anti-Connexin 36 Antibody (C-term) at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36kDa Blocking/Dilution buffer: 5% NFDM/TBST.

The anti-GJA9 C-term Pab is used in Western blot to detect GJA9 in placenta tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.