BD-PB1430-NANOG Rabbit Polyclonal Antibody (S285)-现货抗体产品库武汉枢密脑科学技术有限公司

NANOG Rabbit Polyclonal Antibody (S285)

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产品基本信息

产品货号

BD-PB1430

产品名称

NANOG Rabbit Polyclonal Antibody (S285)

别名

Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog, NANOG

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_079141.2

Human Swissprot No.

Q9H9S0

特异性

This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-292 amino acids from human NANOG.

稀释度

WB~~1:1000;IF~~1:10~50

预测分子量

35kDa

同种型

Rabbit Ig

背景介绍

NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.

组织表达

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

细胞定位

Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000269|PubMed:15983365}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-NANOG Antibody (S285) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of NANOG Antibody (S285) in HepG2 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.

Fluorescent confocal image of SY5Y cells stained with BD-PB1430 NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with BD-PB1430 NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.