BD-PB1425-SCF (KITLG) Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

SCF (KITLG) Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB1425

产品名称

SCF (KITLG) Rabbit Polyclonal Antibody (C-term)

别名

Kit ligand, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, Soluble KIT ligand, sKITLG, KITLG, MGF, SCF

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_000890.1;NP_003985.2

Human Swissprot No.

P21583

特异性

This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the C-terminal region of human SCF (KITLG).

稀释度

WB~~1:1000;IF~~1:10~50;FC~~1:10~50

预测分子量

31kDa

同种型

Rabbit Ig

背景介绍

KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.

细胞定位

[Isoform 1]: Cell membrane; Single-pass type I membrane protein [Soluble KIT ligand]: Secreted.

期货

现货

纯化

Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Western blot analysis of KITLG Antibody (C-term) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab.

Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) .

Immunofluorescence analysis of anti-KITLG Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.