BD-PB1399-ALDH6A1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ALDH6A1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1399

产品名称

ALDH6A1 Rabbit Polyclonal Antibody (N-term)

别名

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, MMSDH, Malonate-semialdehyde dehydrogenase [acylating], Aldehyde dehydrogenase family 6 member A1, ALDH6A1, MMSDH

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_005580.1

Human Swissprot No.

Q02252

特异性

This ALDH6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human ALDH6A1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

58kDa

同种型

Rabbit Ig

背景介绍

ALDH6A1 belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. This protein is a mitochondrial methylmalonate semialdehyde dehydrogenase, and catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

细胞定位

Mitochondrion.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. The antibody is eluted with high and low pH buffers and neutralized immediate

Anti-ALDH6A1 Antibody (N-term) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ALDH6A1 Antibody (N-term) in T47D cell line lysates (35ug/lane). ALDH6A1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with *ALDH6A1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of ATDC5 cells using ALDH6A1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.