BD-PB1394-TBX5 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

TBX5 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1394

产品名称

TBX5 Rabbit Polyclonal Antibody (N-term)

别名

T-box transcription factor TBX5, T-box protein 5, TBX5

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_000183.2;NP_542448.1;NP_852259.1

Human Swissprot No.

Q99593

特异性

This TBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TBX5.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

58kDa

同种型

Rabbit Ig

背景介绍

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

细胞定位

Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201, ECO:0000269|PubMed:29174768}. Cytoplasm Note=Shuttles between the cytoplasm and the nucleus. Acetylation at Lys-339 promotes nuclear retention.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-TBX5 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: HL-60 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

TBX5 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TBX5 antibody detected the TBX5 protein (arrow).

TBX5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBX5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.