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PSMA5 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB1359
产品名称
PSMA5 Rabbit Polyclonal Antibody (N-term)
别名
Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001186701.1;NP_001186702.1;NP_001186703.1;NP_002781.2
Human Swissprot No.
P28066
特异性
This PSMA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PSMA5.
稀释度
WB~~1:1000;IHC-P~~1:100~500
预测分子量
26kDa
同种型
Rabbit Ig
背景介绍
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit.
组织表达
Expressed in fetal brain (at protein level).
细胞定位
Cytoplasm. Nucleus
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Western blot analysis of PSMA5 (arrow) using rabbit polyclonal PSMA5 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2) .

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PSMA5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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