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Mouse Csf1r Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB1248
产品名称
Mouse Csf1r Rabbit Polyclonal Antibody (C-term)
别名
Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, Csf1r, Csfmr, Fms
类别
常规抗体
推荐应用
WB
反应种属
Rat, Mouse
Human Swissprot No.
P09581
Mouse Gene ID
NP_001032948.2
特异性
This Mouse Csf1r antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 895-923 amino acids from the C-terminal region of mouse Csf1r.
稀释度
IF~~1:25;WB~~1:2000
预测分子量
109kDa
同种型
Rabbit Ig
背景介绍
Csf1r is a protein tyrosine-kinase transmembrane receptor for CSF1 and IL34.
组织表达
Widely expressed..
细胞定位
Cell membrane; Single-pass type I membrane protein. Note=The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r with BD-PB1248 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).

All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:2000 dilution Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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