Mouse Csf1r Rabbit Polyclonal Antibody (C-term)
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r with BD-PB1248 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
All lanes : Anti-Mouse Csf1r Antibody (C-term) at 1:2000 dilution Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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