BD-PB1197-TUBA4A Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

TUBA4A Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB1197

产品名称

TUBA4A Rabbit Polyclonal Antibody (C-term)

别名

Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A, TUBA1

类别

常规抗体

推荐应用

反应种属

Human, Mouse, Rat

Human Gene ID

NP_001265481.1;NP_005991.1

Human Swissprot No.

P68366

特异性

This TUBA4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 417-446 amino acids from the C-terminal region of human TUBA4A.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

50kDa

同种型

Rabbit Ig

背景介绍

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.

细胞定位

Cytoplasm, cytoskeleton.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-TUBA4A Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

TUBA4A Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the TUBA4A antibody detected the TUBA4A protein (arrow).

TUBA4A Antibody (C-term)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBA4A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.