BD-PB1171-PI15 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

PI15 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1171

产品名称

PI15 Rabbit Polyclonal Antibody (N-term)

别名

Peptidase inhibitor 15, PI-15, 25 kDa trypsin inhibitor, p25TI, Cysteine-rich secretory protein 8, CRISP-8, SugarCrisp, PI15, CRISP8, P25TI

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_056970.1

Human Swissprot No.

O43692

特异性

This PI15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-63 amino acids from the N-terminal region of human PI15.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

29kDa

同种型

Rabbit Ig

背景介绍

This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq].

组织表达

Weakly expressed. Expressed at low level in prostate, mammary gland, salivary gland and thyroid gland

细胞定位

Secreted.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-PI15 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

PI15 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PI15 antibody detected the PI15 protein (arrow).

PI15 Antibody (N-term)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PI15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

PI15 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.