BD-PB1145-PCDH9 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

PCDH9 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB1145

产品名称

PCDH9 Rabbit Polyclonal Antibody (C-term)

别名

Protocadherin-9, PCDH9

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_065136.1;NP_982354.1

Human Swissprot No.

Q9HC56

特异性

This PCDH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1018-1046 amino acids from the C-terminal region of human PCDH9.

稀释度

IHC-P-Leica~~1:500;WB~~1:2000

预测分子量

136kDa

同种型

Rabbit Ig

背景介绍

This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The mRNA encodes a cadherin-related neuronal receptor that localizes to synaptic junctions and is putatively involved in specific neuronal connections and signal transduction. Sharing a characteristic with other protocadherin genes, this gene has a notably large exon that encodes six cadherin domains and a transmembrane region. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

细胞定位

Cell membrane; Single-pass type I membrane protein

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using BD-PB1145 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human brain tissue using BD-PB1145 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-PCDH9 Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.