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mouse BID Rabbit Polyclonal Antibody (S61)

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产品基本信息

产品货号
BD-PB1133
产品名称
mouse BID Rabbit Polyclonal Antibody (S61)
别名
BH3-interacting domain death agonist, p22 BID, BID, BH3-interacting domain death agonist p15, p15 BID, BH3-interacting domain death agonist p13, p13 BID, BH3-interacting domain death agonist p11, p11 BID, Bid
类别
常规抗体
推荐应用
WB
反应种属
Mouse
Human Swissprot No.
P70444
Mouse Gene ID
NP_031570.2
特异性
This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from mouse BID.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
22kDa
同种型
Rabbit Ig
背景介绍
Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release.
细胞定位
Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane {ECO:0000250|UniProtKB:P55957}. Note=When uncleaved, it is predominantly cytoplasmic. [BH3-interacting domain death agonist p13]: Mitochondrion membrane. Note=Associated with the mitochondrial membrane.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of anti-mouse BID Antibody (S61) in mouse spleen tissue lysates (35ug/lane). BID (arrow) was detected using the purified Pab .

mouse BID Antibody (S61) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry. Clinical relevance has not been evaluated.

mouse BID Antibody (S61) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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