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MPP3 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB1128
产品名称
MPP3 Rabbit Polyclonal Antibody (C-term)
别名
MAGUK p55 subfamily member 3, Discs large homolog 3, Protein MPP3, MPP3, DLG3
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_001923.2
Human Swissprot No.
Q13368
特异性
This MPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-434 amino acids from the C-terminal region of human MPP3.
稀释度
WB~~1:1000;IF~~1:10~50;FC~~1:10~50
预测分子量
66kDa
同种型
Rabbit Ig
背景介绍
This gene product is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. This protein contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction. Alternatively spliced transcript variants have been identified. One transcript variant is experimentally supported, but it doesn't encode a protein.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-MPP3 Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

MPP3 Antibody (C-term) western blot analysis in K562,NCI-H292 cell line lysates (35ug/lane).This demonstrates the MPP3 antibody detected the MPP3 protein (arrow).

Confocal immunofluorescent analysis of MPP3 Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

MPP3 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

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