BD-PB1110-SFRP5 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

SFRP5 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB1110

产品名称

SFRP5 Rabbit Polyclonal Antibody (Center)

别名

Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_003006.2

Human Swissprot No.

Q5T4F7

特异性

This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the Central region of human SFRP5.

稀释度

IHC-P-Leica~~1:500;WB~~1:2000

预测分子量

36kDa

同种型

Rabbit Ig

背景介绍

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq].

组织表达

Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle

细胞定位

Secreted.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using BD-PB1110 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes : Anti-SFRP5 Antibody (Center) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human pancreas lysate Lane 3: Hela whole cell lysate Lane 4: Mouse eyeball lysate Lane 5: Mouse pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.