BD-PB1106-GSTT1 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GSTT1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1106

产品名称

GSTT1 Rabbit Polyclonal Antibody (N-term)

别名

Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1, GSTT1

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_000844.2

Human Swissprot No.

P30711

特异性

This GSTT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human GSTT1.

稀释度

WB~~1:1000;FC~~1:10~50

预测分子量

27kDa

同种型

Rabbit Ig

背景介绍

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries.

组织表达

Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.

细胞定位

Cytoplasm.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-GSTT1 Antibody (N-term)at 1:2000 dilution + human lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of GSTT1 (arrow) using rabbit polyclonal GSTT1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTT1 gene.

GSTT1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.