BD-PB1066-EIF3CL Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

EIF3CL Rabbit Polyclonal Antibody (N-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB1066

产品名称

EIF3CL Rabbit Polyclonal Antibody (N-term)

别名

Eukaryotic translation initiation factor 3 subunit C {ECO:0000255|HAMAP-Rule:MF_03002}, eIF3c {ECO:0000255|HAMAP-Rule:MF_03002}, Eukaryotic translation initiation factor 3 subunit 8 {ECO:0000255|HAMAP-Rule:MF_03002}, eIF3 p110 {ECO:0000255|HAMAP-Rule:MF_03002}, EIF3C {ECO:0000255|HAMAP-Rule:MF_03002}

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001032897.1;NP_001186071.1;NP_001254503.1;NP_003743.1

Human Swissprot No.

Q99613

特异性

This EIF3CL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-155 amino acids from the N-terminal region of human EIF3CL.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

105kDa

同种型

Rabbit Ig

背景介绍

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

细胞定位

Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03002}.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-EIF3CL Antibody (N-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 105 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

EIF3CL Antibody (N-term)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3CL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

EIF3CL Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.