BD-PB1022-FADS2 Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

FADS2 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB1022

产品名称

FADS2 Rabbit Polyclonal Antibody (N-term)

别名

Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001268430.1;NP_001268431.1;NP_004256.1

Human Swissprot No.

O95864

特异性

This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human FADS2.

稀释度

FC~~1:25;WB~~1:1000

预测分子量

52kDa

同种型

Rabbit Ig

背景介绍

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

组织表达

Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.

细胞定位

Endoplasmic reticulum membrane; Multi-pass membrane protein

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Overlay histogram showing HepG2 cells stained with BD-PB1022(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-FADS2 Antibody (N-term) at 1:1000 dilution Lane 1: Human breast lysate Lane 2:Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.