BD-PB1010-TCRB Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

TCRB Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB1010

产品名称

TCRB Rabbit Polyclonal Antibody (Center)

别名

T-cell receptor beta chain V region CTL-L17, TCRB

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

P04435

特异性

This TCRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-94 amino acids from the Central region of human TCRB.

稀释度

WB~~1:1000

预测分子量

13kDa

同种型

Rabbit Ig

背景介绍

The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.

细胞定位

Cell membrane.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-TCRB Antibody (Center)at 1:1000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MOLT-4 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-TCRB Antibody (Center)at 1:500 dilution + MDA-MB-231 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-TCRB Antibody (Center) at 1:1000 dilution + human spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size :15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

TCRB Antibody (Center) western blot analysis in MDA-MB231,293,Ramos,NCI-H460 cell line lysates (35ug/lane).This demonstrates the TCRB antibody detected the TCRB protein (arrow).