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MSI1 Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB0984
产品名称
MSI1 Rabbit Polyclonal Antibody (N-term)
别名
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_002433.1
Human Swissprot No.
O43347
特异性
This MSI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-93 amino acids from the N-terminal region of human MSI1.
稀释度
WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50
预测分子量
39kDa
同种型
Rabbit Ig
背景介绍
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
组织表达
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines
细胞定位
Cytoplasm {ECO:0000250|UniProtKB:Q61474}. Nucleus {ECO:0000250|UniProtKB:Q61474}
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of lysate from SH‑SY5Y cell line, using MSI1 Antibody (N-term)at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

MSI1 Antibody (N-term)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MSI1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

MSI1 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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