Immunohistochemical analysis of BD-PB0962 on paraffin-embedded human testis tissue was performed on the Leica??BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

All lanes : Anti-VSIG8 Antibody (C-term) at 1:2000 dilution Lane 1: RD whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.