BD-PB0950-UQCRFS1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

UQCRFS1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB0950

产品名称

UQCRFS1 Rabbit Polyclonal Antibody (C-term)

别名

Cytochrome b-c1 complex subunit Rieske, mitochondrial, Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, RISP, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Cytochrome b-c1 complex subunit 11, Complex III subunit IX, Ubiquinol-cytochrome c reductase 8 kDa protein, UQCRFS1

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_005994.2

Human Swissprot No.

P47985

特异性

This UQCRFS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-209 amino acids from the C-terminal region of human UQCRFS1.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50;IF~~1:10~50

预测分子量

30kDa

同种型

Rabbit Ig

背景介绍

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex (By similarity).

细胞定位

Mitochondrion inner membrane; Single-pass membrane protein {ECO:0000250|UniProtKB:Q5ZLR5}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-UQCRFS1 Antibody (C-term) at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

UQCRFS1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UQCRFS1 antibody detected the UQCRFS1 protein (arrow).

UQCRFS1 Antibody (C-term)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UQCRFS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

UQCRFS1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of UQCRFS1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).