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FUT8 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号
BD-PB0886
产品名称
FUT8 Rabbit Polyclonal Antibody (Center)
别名
Alpha-(1,6)-fucosyltransferase, Alpha1-6FucT, Fucosyltransferase 8, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase, GDP-fucose--glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase, FUT8
类别
常规抗体
推荐应用
WB
反应种属
Human
Human Gene ID
NP_004471.4;NP_835368.1;NP_835369.1
Human Swissprot No.
Q9BYC5
特异性
This FUT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-357 amino acids from the Central region of human FUT8.
稀释度
WB~~1:1000;IHC-P~~1:100~500
预测分子量
67kDa
同种型
Rabbit Ig
背景介绍
This enzyme belongs to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternatively spliced variants encoding different isoforms have been identified.
细胞定位
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein Note=Membrane-bound form in trans cisternae of Golgi.
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

All lanes : Anti-FUT8 Antibody (Center) at 1:2000 dilution Lane 1: MDA-MB-231 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size : 65kDa Blocking/Dilution buffer: 5% NFDM/TBST.

FUT8 Antibody (Center)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FUT8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

FUT8 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the FUT8 antibody detected the FUT8 protein (arrow).

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