BD-PB0885-GAD2 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

GAD2 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB0885

产品名称

GAD2 Rabbit Polyclonal Antibody (Center)

别名

Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_000809.1;NP_001127838.1

Human Swissprot No.

Q05329

特异性

This GAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human GAD2.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

65kDa

同种型

Rabbit Ig

背景介绍

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

细胞定位

Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody (Center). BD-PB0885 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

GAD2 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow).

GAD2 Antibody (Center)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of GAD2 Antibody (Center)with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).