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IFITM5 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号
BD-PB0878
产品名称
IFITM5 Rabbit Polyclonal Antibody (Center)
别名
Interferon-induced transmembrane protein 5, Bone-restricted interferon-induced transmembrane protein-like protein, BRIL, Dispanin subfamily A member 1, DSPA1, IFITM5
类别
常规抗体
推荐应用
WB
反应种属
Human, Mouse
Human Gene ID
NP_001020466.1
Human Swissprot No.
A6NNB3
特异性
This IFITM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the Central region of human IFITM5.
稀释度
FC~~1:25;WB~~1:4000
预测分子量
14kDa
同种型
Rabbit Ig
背景介绍
Plays a role in bone mineralization (By similarity).
组织表达
Detected in bone (PubMed:24058703). Detected in osteoblasts and fibroblasts (at protein level) (PubMed:24519609) Detected in bone (PubMed:24058703). Detected in osteoblasts and fibroblasts (PubMed:24519609).
细胞定位
Cell membrane; Multi-pass membrane protein
期货
现货
纯化
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Overlay histogram showing A431 cells stained with BD-PB0878(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

All lanes : Anti-IFITM5 Antibody (Center) at 1:4000 dilution Lane 1: T47D whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: SW480 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: PANC-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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