产品名称
JAM3 Rabbit Polyclonal Antibody
蛋白名称
Junctional adhesion molecule C (JAM-C) (JAM-2) (Junctional adhesion molecule 3) (JAM-3)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q9BX67
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q9BX67/entry
Mouse Swissprot No.
Q9D8B7
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q9D8B7
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q68FQ2
免疫原
Synthesized peptide derived from human protein . at AA range: 60-140
特异性
JAM3 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq,
组织表达
Detected on round and elongated spermatids (at protein level) (PubMed:15372036). Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum (at protein level). Also detected in the synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis or ostearthritis (at protein level).
细胞定位
Cell membrane ; Single-pass type I membrane protein . Cell junction . Cell junction, desmosome . Cell junction, tight junction . Detected in the acrosome region in developing spermatids (By similarity). In epithelial cells, it is expressed at desmosomes but not at tight junctions (PubMed:15194813). Localizes at the cell surface of endothelial cells; treatment of endothelial cells with vascular endothelial growth factor stimulates recruitment of JAM3 to cell-cell contacts (PubMed:15994945). .; [Soluble form of JAM-C]: Secreted .
信号通路
Cell adhesion molecules (CAMs);Tight junction;Leukocyte transendothelial migration;Epithelial cell signaling in Helicobacter pylori infection;
功能
function:May participate in cell-cell adhesion distinct from tight junctions.,similarity:Belongs to the immunoglobulin superfamily.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subunit:Interacts with JAM2.,tissue specificity:Widely expressed. Highest expression in placenta, brain and kidney.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
