BD-PB0811-ACF Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ACF Rabbit Polyclonal Antibody (C-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB0811

产品名称

ACF Rabbit Polyclonal Antibody (C-term)

别名

APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312|EMBL:CAB947541}

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001185747.1;NP_001185748.1;NP_001185749.1;NP_055391.2;NP_620310.1;NP_620311.1

Human Swissprot No.

Q9NQ94

特异性

This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

65kDa

同种型

Rabbit Ig

背景介绍

Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.

组织表达

Widely expressed with highest levels in brain, liver, pancreas, colon and spleen.

细胞定位

Nucleus. Endoplasmic reticulum. Cytoplasm. Note=Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

ACF Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the ACF antibody detected the ACF protein (arrow).

ACF Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ACF Antibody (C-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.