BD-PB0808-UBC9 (UBE2I) Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

UBC9 (UBE2I) Rabbit Polyclonal Antibody (N-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB0808

产品名称

UBC9 (UBE2I) Rabbit Polyclonal Antibody (N-term)

别名

SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_003336.1;NP_919235.1;NP_919236.1;NP_919237.1

Human Swissprot No.

P63279

特异性

This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

18kDa

同种型

Rabbit Ig

背景介绍

UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.

组织表达

Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

细胞定位

Nucleus. Cytoplasm Note=Mainly nuclear (By similarity). In spermatocytes, localizes in synaptonemal complexes (PubMed:8610150). Recruited by BCL11A into the nuclear body (By similarity). {ECO:0000250|UniProtKB:P63280, ECO:0000269|PubMed:8610150}

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Western blot analysis of anti-UBE2I Antibody (N-term) in A2058 cell line lysates (35ug/lane). UBE2I(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of UBE2I (arrow) using rabbit polyclonal UBE2I Antibody (S7) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.