BD-PB0756-PEMT Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

PEMT Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB0756

产品名称

PEMT Rabbit Polyclonal Antibody (N-term)

别名

Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001254480.1;NP_001254481.1;NP_009100.2;NP_680477.1;NP_680478.1

Human Swissprot No.

Q9UBM1

特异性

This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEMT.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

22kDa

同种型

Rabbit Ig

背景介绍

This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms.

细胞定位

[Isoform 1]: Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Note=Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Western blot analysis of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody (N-term). BD-PB0756 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot analysis of anti-PEMT Antibody (N-term) Pab in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.