BD-PB0749-Dnmt2 Rabbit Polyclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Dnmt2 Rabbit Polyclonal Antibody

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产品基本信息

产品货号

BD-PB0749

产品名称

Dnmt2 Rabbit Polyclonal Antibody

别名

tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, MHsaIIP, PuMet, TRDMT1, DNMT2

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_004403.1

Human Swissprot No.

O14717

特异性

This Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from human Dnmt2.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

45kDa

同种型

Rabbit Ig

背景介绍

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms.

组织表达

Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes

细胞定位

Cytoplasm.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti-Dnmt2 Antibody at 1:1000 dilution + mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-hDnmt2-P361 Pab in mouse cerebellum tissue lysates (35ug/lane).hDnmt2-P361(arrow) was detected using the purified Pab (1:60 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.