BD-PB0712-PSMA5(12D2)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PSMA5(12D2)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB0712

产品名称

PSMA5(12D2)Mouse Monoclonal Antibody

别名

Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001186701.1;NP_001186702.1;NP_001186703.1;NP_002781.2

Human Swissprot No.

P28066

特异性

Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody.

稀释度

WB~~1:500~1000;IHC-P~~1:100~500;IF~~1:25

预测分子量

26kDa

同种型

IgG1

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

组织表达

Expressed in fetal brain (at protein level).

细胞定位

Cytoplasm. Nucleus

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

All lanes : Anti-PSMA5 Antibody at 1:1000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Neuro-2a whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody. BD-PB0712 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody. BD-PB0712 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

PSMA5 Antibody western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).