BD-PB0712-PSMA5(12D2)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PSMA5(12D2)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB0712

产品名称

PSMA5(12D2)Mouse Monoclonal Antibody

别名

Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_001186701.1;NP_001186702.1;NP_001186703.1;NP_002781.2

Human Swissprot No.

P28066

特异性

Purified His-tagged PSMA5 protein(Fragment) was used to produced this monoclonal antibody.

稀释度

WB 1:500-1000

预测分子量

26kDa

同种型

IgG1

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit.

组织表达

Expressed in fetal brain (at protein level).

细胞定位

Cytoplasm. Nucleus

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

All lanes : Anti-PSMA5 Antibody at 1:1000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: Neuro-2a whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. testis section using PSMA5 Antibody. BD-PB0712 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of Hela cells stained with XAF1 PSMA5 Antibody. BD-PB0712 was diluted at 1:25 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

PSMA5 Antibody western blot analysis in K562 cell line lysates (35μg/lane).This demonstrates the PSMA5 antibody detected the PSMA5 protein (arrow).