BD-PB0687-ACOX1(10R4)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ACOX1(10R4)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB0687

产品名称

ACOX1(10R4)Mouse Monoclonal Antibody

别名

Peroxisomal acyl-coenzyme A oxidase 1, AOX, Palmitoyl-CoA oxidase, Straight-chain acyl-CoA oxidase, SCOX, ACOX1, ACOX

类别

常规抗体

推荐应用

反应种属

Human

Human Gene ID

NP_001171968.1;NP_004026.2;NP_009223.2

Human Swissprot No.

Q15067

特异性

This ACOX1 monoclonal antibody is generated from mouse immunized with ACOX1 recombinant protein.

稀释度

WB~~1:100-1:1000;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

74kDa

同种型

IgG1

背景介绍

ACOX1 is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids.

组织表达

Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.

细胞定位

Peroxisome.

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Anti- at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ACOX1 Monoclonal Antibodywestern blot analysis in K562 cell line lysates (15μg/lane).This demonstrates the ACOX1 antibody detected the ACOX1 protein (arrow).(1μg/ml)1:390

ACOX1 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACOX1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of ACOX1 Antibody with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).