BD-PB0685-ALDH6A1(1T5)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ALDH6A1(1T5)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB0685

产品名称

ALDH6A1(1T5)Mouse Monoclonal Antibody

别名

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, MMSDH, Malonate-semialdehyde dehydrogenase [acylating], Aldehyde dehydrogenase family 6 member A1, ALDH6A1, MMSDH

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_005580.1

Human Swissprot No.

Q02252

特异性

This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein.

稀释度

IF~~1:25;IHC-P~~1:100~500;WB~~1:1000

预测分子量

58kDa

同种型

IgG1,IgK

背景介绍

This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

细胞定位

Mitochondrion.

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody . BD-PB0685 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody. BD-PB0685 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from MCF-7, T47D cell line (from left to right), using ALDH6A1 Antibody. BD-PB0685 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. BD-PB0685 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.