BD-PB0683-LC3(APG8)(14A17)Mouse Monoclonal Antibody(APG8)-现货抗体产品库武汉枢密脑科学技术有限公司

LC3(APG8)(14A17)Mouse Monoclonal Antibody(APG8)

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产品基本信息

产品货号

BD-PB0683

产品名称

LC3(APG8)(14A17)Mouse Monoclonal Antibody(APG8)

别名

Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_115903.1;NP_852610.1

Human Swissprot No.

Q9H492;Q9GZQ8

特异性

This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

稀释度

WB~~1:1000

同种型

Mouse IgG1 k

背景介绍

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

All lanes : Anti-MAP1LC3A Antibody at dilution Lane 1: Mouse brain whole cell lysate Lane 2: Rat brain whole cell lysate Lane 3: Human brain whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-LC3 Mab at 8 μg/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).

Western blot analysis of anti-LC3 Mab Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.