BD-PB0682-UBC9(12H10)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

UBC9(12H10)Mouse Monoclonal Antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB0682

产品名称

UBC9(12H10)Mouse Monoclonal Antibody

别名

SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

Human Gene ID

NP_003336.1;NP_919235.1;NP_919236.1;NP_919237.1

Human Swissprot No.

P63279

特异性

This UBC9 antibody was raised using purified GST-UBC9 fusion protein.

稀释度

WB~~1:100~500

预测分子量

18kDa

同种型

Mouse IgG1

背景介绍

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

组织表达

Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

细胞定位

Nucleus. Cytoplasm Note=Mainly nuclear (By similarity). In spermatocytes, localizes in synaptonemal complexes (PubMed:8610150). Recruited by BCL11A into the nuclear body (By similarity). {ECO:0000250|UniProtKB:P63280, ECO:0000269|PubMed:8610150}

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

All lanes : Anti-UBC9 Antibody at 1:1000-1:2000 dilution Lane 1: human heart lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-UBC9 Antibody in Ramos cell line lysates (35μg/lane). UBC9(arrow) was detected using the purified Mab.