BD-PB0680-TBB5(15I8)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

TBB5(15I8)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB0680

产品名称

TBB5(15I8)Mouse Monoclonal Antibody

别名

Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5

类别

常规抗体

推荐应用

反应种属

Human, Mouse, Rat

Human Gene ID

NP_821133.1

Human Swissprot No.

P07437

特异性

TBB5 recombinant protein is used to produce this monoclonal antibody.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:25

预测分子量

50kDa

同种型

IgG2b,Igκ

组织表达

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

细胞定位

Cytoplasm, cytoskeleton

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody. BD-PB0680 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody. BD-PB0680 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunofluorescent analysis of A549 cells, using TBB5 Antibody conjugated Phalloidin (red).

Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.