BD-PB0685-ALDH6A1(1T5)Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ALDH6A1(1T5)Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB0685

产品名称

ALDH6A1(1T5)Mouse Monoclonal Antibody

别名

Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, MMSDH, Malonate-semialdehyde dehydrogenase [acylating], Aldehyde dehydrogenase family 6 member A1, ALDH6A1, MMSDH

类别

常规抗体

推荐应用

反应种属

Human, Mouse

Human Gene ID

NP_005580.1

Human Swissprot No.

Q02252

特异性

This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein.

稀释度

WB 1:1000

预测分子量

58kDa

同种型

IgG1,IgK

背景介绍

This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

细胞定位

Mitochondrion.

期货

现货

纯化

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody . BD-PB0685 was diluted at 1:25 dilution. An Alexa Fluor? 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody. BD-PB0685 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from MCF-7, T47D cell line (from left to right), using ALDH6A1 Antibody. BD-PB0685 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.

Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. BD-PB0685 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.