BD-PB0650-ATF4 Rabbit Polyclonal Antibody (S245)-现货抗体产品库武汉枢密脑科学技术有限公司

ATF4 Rabbit Polyclonal Antibody (S245)

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产品基本信息

产品货号

BD-PB0650

产品名称

ATF4 Rabbit Polyclonal Antibody (S245)

别名

Tax-responsive enhancer element B67; ATF4 protein

类别

常规抗体

推荐应用

反应种属

Human

Human Swissprot No.

Q96AQ3

特异性

This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-252 amino acids from human ATF4.

稀释度

WB~~1:1000

宿主

Mouse

同种型

Rabbit Ig

背景介绍

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.

期货

现货

纯化

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anti-ATF4 Antibody (S245) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) .

Western blot analysis of ATF4 Antibody (S245) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.