产品名称
Rev-erbα (phospho-Ser55/59) Rabbit Polyclonal Antibody
别名
Nuclear receptor subfamily 1 group D member 1 (Rev-erbA-alpha) (V-erbA-related protein 1) (EAR-1)
基因名称
NR1D1 EAR1 HREV THRAL
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/9572
Human Swissprot No.
P20393
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P20393/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/217166
Mouse Swissprot No.
Q3UV55
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q3UV55
Rat Gene Link
https://www.uniprot.org/uniprot/252917
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q63503
免疫原
Synthesized phosho peptide around human Rev-erbα (Ser55 and 59)
特异性
This antibody detects endogenous levels of Human Rev-erbα (phospho-Ser55 or 59)
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a transcription factor that is a member of the nuclear receptor subfamily 1. The encoded protein is a ligand-sensitive transcription factor that negatively regulates the expression of core clock proteins. In particular this protein represses the circadian clock transcription factor aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL). This protein may also be involved in regulating genes that function in metabolic, inflammatory and cardiovascular processes. [provided by RefSeq, Jan 2013],
组织表达
Widely expressed. Expressed at high levels in the liver, adipose tissue, skeletal muscle and brain. Also expressed in endothelial cells (ECs), vascular smooth muscle cells (VSMCs) and macrophages. Expression oscillates diurnally in the suprachiasmatic nucleus (SCN) of the hypothalamus as well as in peripheral tissues. Expression increases during the differentiation of pre-adipocytes into mature adipocytes. Expressed at high levels in some squamous carcinoma cell lines.
细胞定位
Nucleus . Cytoplasm . Cell projection, dendrite . Cell projection, dendritic spine . Localizes to the cytoplasm, dendrites and dendritic spine in the presence of OPHN1. Localizes predominantly to the nucleus at ZT8 whereas it is cytoplasmic at ZT20. Phosphorylation by CSNK1E enhances its cytoplasmic localization. .
功能
domain:Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.,function:Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.,similarity:Belongs to the nuclear hormone receptor family. NR1 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with C1D.,tissue specificity:Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.