产品名称
Prdx1 (phospho-Tyr194) Rabbit Polyclonal Antibody
别名
Peroxiredoxin-1 (EC 1.11.1.15) (Natural killer cell-enhancing factor A) (NKEF-A) (Proliferation-associated gene protein) (PAG) (Thioredoxin peroxidase 2) (Thioredoxin-dependent peroxide reductase 2)
基因名称
PRDX1 PAGA PAGB TDPX2
蛋白名称
Prdx1 (Tyr194) ,Peroxiredoxin-1
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/5052
Human Swissprot No.
Q06830
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q06830/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/18477
Mouse Swissprot No.
P35700
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P35700
Rat Gene Link
https://www.uniprot.org/uniprot/117254
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q63716
免疫原
Synthesized phosho peptide around human Prdx1 (Tyr194)
特异性
This antibody detects endogenous levels of Human Prdx1 (phospho-Tyr194)
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011],
组织表达
Brain,Cajal-Retzius cell,Fetal brain cortex,Urinary bladder,
细胞定位
Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
功能
catalytic activity:2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.,function:Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).,induction:Constitutively expressed in most human cells; is induced to higher levels upon serum stimulation in untransformed and transformed cells.,miscellaneous:Inactivated upon oxidative stress by overoxidation of Cys-52 to Cys-SO(2)H and Cys-SO(3)H. Cys-SO(2)H is retroreduced to Cys-SOH after removal of H(2)O(2), while Cys-SO(3)H may be irreversibly oxidized.,miscellaneous:The active site is the redox-active Cys-52 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-173-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin.,PTM:Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.,similarity:Belongs to the ahpC/TSA family.,similarity:Contains 1 thioredoxin domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer; disulfide-linked, upon oxidation (By similarity). May form heterodimers with AOP2.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
