产品名称
PRAS40 (phospho-Ser183) Rabbit Polyclonal Antibody
别名
Proline-rich AKT1 substrate 1 (40 kDa proline-rich AKT substrate)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/84335
Human Swissprot No.
Q96B36
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96B36/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/67605
Mouse Swissprot No.
Q9D1F4
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9D1F4
免疫原
Synthesized phosho peptide around human PRAS40 (Ser183)
特异性
This antibody detects endogenous levels of Human Mouse PRAS40 (phospho-Ser183)
稀释度
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],
组织表达
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
细胞定位
Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .
功能
function:May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.,subcellular location:Found in the cytosolic fraction of the brain.,subunit:The phosphorylated form interacts with 14-3-3.,tissue specificity:Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
