产品名称
PNK1 (phospho-Ser114/Thr118) Rabbit Polyclonal Antibody
别名
Bifunctional polynucleotide phosphatase/kinase (DNA 5'-kinase/3'-phosphatase) (Polynucleotide kinase-3'-phosphatase) [Includes: Polynucleotide 3'-phosphatase (EC 3.1.3.32) (2'(3')-polynucleotidase); Polynucleotide 5'-hydroxyl-kinase (EC 2.7.1.78)]
蛋白名称
PNK1 (Ser114/Thr118)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/11284
Human Swissprot No.
Q96T60
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96T60/entry
Mouse Swissprot No.
Q9JLV6
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9JLV6
免疫原
Synthesized phosho peptide around human PNK1 (Ser114 and Thr118)
特异性
This antibody detects endogenous levels of Human PNK1 (phospho-Ser114 or Thr118)
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010],
组织表达
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
细胞定位
Nucleus . Chromosome . Localizes to site of double-strand breaks. .
功能
catalytic activity:A 3'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate.,catalytic activity:ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.,function:Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:In the N-terminal section; belongs to the DNA 3' phosphatase family.,tissue specificity:Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
