产品名称
PAK2 (phospho-Ser20) Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Swissprot No.
Q13153
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q13153 /entry
免疫原
Synthesized phosho peptide around human PAK2 (Ser20)
特异性
This antibody detects endogenous levels of Human Mouse PAK2 (phospho-Ser20)
宿主
Polyclonal, Rabbit,IgG
组织表达
Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).
细胞定位
Cytoplasm . Cell junction, focal adhesion . Cell projection, lamellipodium . Cell membrane . Cell projection, ruffle membrane . Cell projection, invadopodium . Nucleus, nucleoplasm . Chromosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Upon DNA damage, translocates to the nucleoplasm when phosphorylated at Thr-212 where is co-recruited with MORC2 on damaged chromatin (PubMed:23260667). Localization to the centrosome does not depend upon the presence of gamma-tubulin (PubMed:27012601). Localization of the active, but not inactive, protein to the adhesions and edge of lamellipodia is mediated by interaction with GIT1 (PubMed:11896197). .
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
