产品名称
CDC20 (phospho-Ser51) Rabbit Polyclonal Antibody
别名
Cell division cycle protein 20 homolog (p55CDC)
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/991
Human Swissprot No.
Q12834
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q12834/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/107995
Mouse Swissprot No.
Q9JJ66
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9JJ66
Rat Gene Link
https://www.uniprot.org/uniprot/64515
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q62623
免疫原
Synthesized phosho peptide around human CDC20 (Ser51)
特异性
This antibody detects endogenous levels of Human Mouse CDC20 (phospho-Ser51)
宿主
Polyclonal, Rabbit,IgG
背景介绍
CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008],
组织表达
Colon,Colon adenocarcinoma,Liver,Lymph,Muscle,Ovary,Skin,Spleen,Testis,
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .
信号通路
Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;Oocyte meiosis;Ubiquitin mediated proteolysis;
功能
developmental stage:Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.,function:Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated during mitosis, probably by maturation promoting factor (MPF).,PTM:Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint.,similarity:Belongs to the WD repeat CDC20/Fizzy family.,similarity:Contains 7 WD repeats.,subunit:Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 (By similarity). Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
