产品名称
CaMKK2 (phospho-Ser511) Rabbit Polyclonal Antibody
别名
Calcium/calmodulin-dependent protein kinase kinase 2 (CaM-KK 2) (CaM-kinase kinase 2) (CaMKK 2) (EC 2.7.11.17) (Calcium/calmodulin-dependent protein kinase kinase beta) (CaM-KK beta) (CaM-kinase kinase beta) (CaMKK beta)
基因名称
CAMKK2 CAMKKB KIAA0787
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/10645
Human Swissprot No.
Q96RR4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96RR4/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/207565
Mouse Swissprot No.
Q8C078
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8C078
Rat Gene Link
https://www.uniprot.org/uniprot/83506
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/O88831
免疫原
Synthesized phosho peptide around human CaMKK2 (Ser511)
特异性
This antibody detects endogenous levels of Human Mouse Rat CaMKK2 (phospho-Ser511)
宿主
Polyclonal, Rabbit,IgG
背景介绍
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012],
组织表达
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
细胞定位
Nucleus . Cytoplasm . Cell projection, neuron projection . Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction. .
功能
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.,domain:The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca(2+)/calmodulin. In part, activity is independent on Ca(2+)/calmodulin.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.,PTM:Autophosphorylated.,sequence caution:Intron retention.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with calmodulin.,tissue specificity:Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.,
纯化
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
