产品名称
PIWL4 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/143689
Human Swissprot No.
Q7Z3Z4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q7Z3Z4/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/330890
Mouse Swissprot No.
Q8CGT6
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q8CGT6
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/Q4G033
免疫原
Synthesized peptide derived from human PIWL4 AA range: 108-158
特异性
This antibody detects endogenous levels of PIWL4 at Human/Mouse/Rat
稀释度
WB 1:500-2000;IHC-p 1:50-300
宿主
Polyclonal, Rabbit,IgG
背景介绍
PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008],
组织表达
Ubiquitously expressed (PubMed:25038252, PubMed:17544373, PubMed:28025795, PubMed:28711973, PubMed:22483988). Detected in retina, retinal pigment epithelia cells (RPE) (at protein level) (PubMed:28025795).
细胞定位
Nucleus . Cytoplasm . Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity). .
功能
function:Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.,similarity:Belongs to the argonaute family. Piwi subfamily.,similarity:Contains 1 PAZ domain.,similarity:Contains 1 Piwi domain.,subcellular location:Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.,tissue specificity:Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
