产品名称
DAD1 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/1603
Human Swissprot No.
P61803
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P61803/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/13135
Mouse Swissprot No.
P61804
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P61804
Rat Gene Link
https://www.uniprot.org/uniprot/192275
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P61805
免疫原
Synthesized peptide derived from human DAD1 AA range: 38-88
特异性
This antibody detects endogenous levels of DAD1 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008],
细胞定位
Endoplasmic reticulum membrane; Multi-pass membrane protein .
功能
catalytic activity:Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.,function:Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis.,similarity:Belongs to the DAD/OST2 family.,subunit:Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
